:: Posted by avi_wener on 04-01-2010
As discussed in an earlier post on siRNA delivery, Bio-Rad’s Gene Pulser MXCell Electroporator is a powerful tool for biotechnology labs involved in delivering siRNA into difficult to transfect cells. In this technical note, biotechnology experts from the University of Rochester demonstrate how the MXCell can be used to simultaneously optimize electroporation conditions in multiple cell types thereby saving time, reagents and effort.
Electroporation of Smooth Muscle Cells Using the Gene Pulser MXcell Electroporation System
:: Posted by avi_wener on 03-31-2010
Small interfereing RNA (siRNA) technology is a powerful tool for facilitating post-transcriptional gene silencing. As with most transfection techniques, siRNAs have been traditionally delivered into the cell by one of four transfection techniques: lipid-mediated, chemical-mediated, viral transfection or electroporation.
In this paper, a team from the Beckman Research Institue of the City of Hope demonstrate how Bio-Rad’s MXCell electroporator can be used to optimize transfection conditions for B-Cell lymphomas which are extremely difficlut to transfect by most traditional methods.
Delivering siRNA into Difficult to Transfect Cells Using Bio-Rad’s Gene Pulser MXcell Electroporation System
