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Quantitative PCR (qPCR): Hallmarks of an Optimized SYBR Green I Assay

If you are currently doing quantitative PCR (qPCR) or plan on beginning qPCR experiments you MUST check out this latest video tutorial from Bio-Rad’s tech support group.

This tutorial describes the characteristics of an optimized SYBR green I quantitative PCR (qPCR) assay. qPCR assays must be optimized to ensure results that are biologically and statistically significant. Topics include a brief review of qPCR chemistry, with an emphasis on SYBR Green I reactions, and definitions of the four main characteristics, or hallmarks of an optimized qPCR assay.

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This entry was posted on Tuesday, February 16th, 2010 at 12:00 pm and is filed under Bio-Rad Tutorial, Videos, qPCR tutorial. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own site.

2 Responses to “Quantitative PCR (qPCR): Hallmarks of an Optimized SYBR Green I Assay”

  1. Bio-Rad Tutorial « Canadian BioTechnologist2.0 says:
    May 26, 2010 at 2:04 pm

    [...] Prior to watching this tutorial, it is recommended that you watch the qPCR-Hallmarks of an optimized SYBR Green I assay first. [...]

  2. Bio-Rad Tutorial: Validating a SYBR Green I Assay « Canadian BioTechnologist2.0 says:
    May 31, 2010 at 7:50 am

    [...] to watching this tutorial, it is recommended that you watch the qPCR-Hallmarks of an optimized SYBR Green I assay [...]

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