Obtaining high quality chemiluminescent blot images with little optimization is a challenge when dealing with a wide range of protein loads and expressions.
Bio-Rad’s new Clarity western ECL substrate provides high sensitivity (see Figure below), long signal duration, and low background even at long exposures. This combination of bright, long signal with low background makes it the perfect choice for routine use on film and digital imagers, even when re-imaging and multiple exposures are required.
The Clarity western ECL substrate is compatible with any HRP-conjugated secondary antibody and is offered in 200 and 500 ml size kits.