Following fast on the heels of last week’s ugly acrylamide mess, here is a submission from one of our Canadian friends. Adriana from the University of Toronto is hoping that you can help her solve the mystery of this warped wonder. Once again, here are the questions:
1. Why is this gel so ugly?
2. How did it get this way?
3. How can Adriana prevent this from happening again?
To help Adriana, simply leave a comment by clicking on the comments link below or fill out the ugly gel feedback form. If you choose to fill out the form, you will be eligible to receive a free TGX sample gel compliments of Bio-Rad Laboratories.
Tags: 2D Gel Electrophoresis, ugly gels
It looks to me like the gel did not set smoothly. Possiable knocked while setting or started to set prior to pouring, causing ripples to form.
There maybe within the gel a electro magnetic polarization of molecules responding to natural magnetic fields that developed while curing. A molecular analysis studying the differentiation between the dark and light areas.
I hope this helped.
Kirk
Looks like a protein overload first. Then it looks like it was terribly over heated due to the use of the wrong buffer in the wrong gel resulting in high resistance, boiling buffer and melting gel.